As abbess of the Convent of the Mother of God, at Pescia, she had a relationship with one of her nuns, Sister Bartolomea. These came to the attention of the counter-reformation papacy, determined to subordinate potentially troublesome mystics if they showed any signs of independent or heretical spirituality. Although they paid three to four visits to the nunnery, it was not until they interrogated Sister Bartolomea that they found that Benedetta and Bartolemea had engaged in sexual relations. Bartolomea gave testimony that Benedetta engaged in frottage with her while possessed by the spirit of a male demon known as Splenditello. Benedetta was stripped of her rank and imprisoned.